Evaluation of the Criterion Stain Free Gel Imaging System for Use in Western Blotting Applications
نویسنده
چکیده
immunodetection is performed. Use of the Criterion Stain Free system eliminates the need for the timeand reagentconsuming traditional methods such as Ponceau S staining used to asses protein transfer prior to detection in western blotting applications. We also demonstrate that, while binding of some monoclonal antibodies may be affected by the Criterion Stain Free method, we have not observed an effect on antigen recognition by polyclonal antibodies with the Criterion Stain Free method.
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